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KMID : 1041219760180020089
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Journal of Sericultural and Entomological Science
1976 Volume.18 No. 2 p.89 ~ p.93
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Studies on the Effect of Cation on the Activity at the 5th Instar Larvae of Bombyx mori
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Yoon Chong-Kwan
Sa Ki-Yon
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Abstract
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The physiological saline solution for animals is known as Ringer’s solution which is used for keeping the function of cold blooded vertebrate animals. Primaily the saline solution is used for the purpose of perfusion experiment in frogs. Later the saline solution is applied in several kinds of animals including human being with satisfactory results. However, this saline solution was introduced to silkworm and it was found that the result was not as successful as in the case of other animals and human being. Normally, in the case of silkworm, the physiological saline solution is prepared in order to maintain the normal function of separated organs and tissues. To this end, the saline solution is adjusted to contain the certain amount and strength of ions, osmosis pressure and hydrogen concentration. The most of cases, the physiological saline solution should be prepared so that the constituent of the solution be the same with the blood selium and body fluid. The hydrogen concentration in the ion element of the saline solution is adjustable by adding Na＋/, K＋/, Ca＋＋/, Mg＋＋/ which are followed by adding of buffer solution such as NaHCOand NaHPO. Determination of optimum concentration of cation in the physiological saline solution, and the optimum mixing rate of more than two kinds of cations are based on the movement of dorsal vessel in the silkworm larvae. The optimum concentration of cations in the solution is prepared by adding NaCl solution which is under zero point. However, this solution was further added with the different concentration of KCl and CaCl. By dropping the prepared solution on the 5th larvae, the effects of solution was measured. The measurement was done by observation of movement’ of dorsal vessel and its time length, and the number of pulses. According to the experiment, it was found that when only NaCl solution was applied, the number of pulses is increased for a moment, and the pulse stopped after one hour or so. When KCl solution was added the time of pulse was prolonged and in the contrast, the number of pulses was slow down. If KCl and CaClsolutions are added the time of pulse was further prolonged. Even though the adding of KCl and CaClare found to be effectible, the correlation between the concentration of solution and the movement of dorsal vessel was not observed. However, it was same in the case of adding Ca＋＋/ or K＋/. It was found that when Mg＋＋/ was added to dorsal vessel the number of the pulses was not decreased although the prolonged time pulse was observed.
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